Elhaddaoui, A, Merlin, J. C., Delacourte, A. and Turrell, S (1992) “Spectroscopic studies of the effect of pH on the interaction between congo-red and amyloid-type proteins”. Aspergillus niger was isolated from processing wastewater [9]. 2.5.1. This is possible through several extracellular lignin-degrading enzymes [6], such as lignin peroxidase, manganese-dependent peroxidase, and laccase. Therefore, the CR degradation seems to detoxify the azo dye. We will be providing unlimited waivers of publication charges for accepted research articles as well as case reports and case series related to COVID-19. When the speed increased from 150 to 200 rpm, the MnP activity decreased but LiP activity remained almost constant. The maximum removal efficiency was recorded at agitation speed of 250 rpm, after which no significant increase was noticed. The objective of this study was to assess the congo red biodegradation and detoxification by Aspergillus niger . Copyright © 2018 Nedra Asses et al. A total decolorization of Procion Red MX-5B by Aspergillus niger was obtained after 336 h of treatment. The presence of ligninolytic extracellular enzymes in culture supports biological decolorization alongside the nonbiological color removal by adsorption [19]. This result is in accordance with the deaminating effect of GlcN6P deaminase in A. niger observed in LC-MS/MS data. Congo red is the most common dyes that can be found in textile industry. Decolorization, biomass production, pH, LiP, and MnP activities were monitored during 6 days (Figure 2). Congo red bioconversion formed degradation metabolites mainly by peroxidases activities, i.e., the sodium naphthalene sulfonate (m/z = 227) and the cycloheptadienylium (m/z = 91). Color reduction was assayed by absorbance measurement at =495nm nm using a Jenway 3540 UV/VIS spectrophotometer. This dye is known to be metabolized into benzidine, which is a human carcinogen and mutagen; that is why it is banned in many countries. as a result of better oxygen transfer and nutrient distribution through the medium. Physical property data is provided to ChemIDplus by SRC, Inc. An acid dye used in testing for hydrochloric acid in gastric contents. We are committed to sharing findings related to COVID-19 as quickly as possible. Effect of initial dye shaking (a), dye concentration (b), pH (c), and temperature (d) on CR decolorization efficiency (Gray colored bars), LiP (□), and MnP activities (∆). The degradation of the CR dye may occur via the following steps: (i) the simultaneous total deamination and oxygenation of (CR) forming the compound (A) with m/z value of 698. Another stain that produces red cell nuclei. Congo red is an organic compound, the sodium salt of 3,3′-([1,1′-biphenyl]-4,4′-diyl)bis(4-aminonaphthalene-1-sulfonic acid).It is an azo dye.Congo red is water-soluble, yielding a red colloidal solution; its solubility is greater in organic solvents. When the speed increases from 0 to 150 rpm, the decolorization efficiency increases from 45% to 98% after six days of culture, indicating that shaking increases the mixing of the oxygen present in the medium, thus helping Aspergillus niger growth (Figure 1(a)). Office of Toxic Substances Report. Incubation temperature was 30°C and 37°C for B. cereus and E. coli, respectively. On the contrary, some authors noticed decolorization decreased under shaking condition due to the competition between azo dyes and oxygen for reduced electron carriers [16]. 1, and washed with sterile distilled water. Effects were considered significant when the P value was < 0.05. Isolated from Ladakh, India,”, M. S. Mahmoud, M. K. Mostafa, S. A. Mohamed, N. A. Sobhy, and M. Nasr, “Bioremediation of red azo dye from aqueous solutions by Aspergillus niger strain isolated from textile wastewater,”, S. D. Kalme, G. K. Parshetti, S. U. Jadhav, and S. P. Govindwar, “Biodegradation of benzidine based dye Direct Blue-6 by Pseudomonas desmolyticum NCIM 2112,”, G. K. Parshetti, S. D. Kalme, S. S. Gomare, and S. P. Govindwar, “Biodegradation of Reactive blue-25 by Aspergillus ochraceus NCIM-1146,”, B.-E. Wang and Y.-Y. The experiment was conducted using Zea mais and Solanum lycopersicum seeds. Safranin. The maximum mycelium dry weight obtained was 6.4 g.L−1. The FTIR spectra were then recorded between 4000 and 400 cm−1, at a rate of 16 nm/s. Decolorization rate was determined according to the following formulation: is the dye absorbance before decolorization and is the dye absorbance after decolorization. An analysis of variance (a one-way ANOVA) was conducted by employing performed (SPSS) version 16.0 software. Kelenyi, G (1967) “Thioflavin S fluorescent and Congo red anisotropic stainings in the histologic demonstration of amyloid”, Delacourte, A., Defossez, A., Persuy, P. and Peers, M. C (1987) “Obbservation of morphological relationship between angiopathic blood vessels and degenerative neurites in Alzheimer’s disease”. Bacillus cereus ATCC 11778 and Escherichia coli ATCC 10536 strains were used for toxicity evaluation of the untreated and treated RC solution by Aspergillus niger. LiP activity was detected after 2 days and reached a maximum at the 5th of the culture. Kang, J., Lemaire, H-G., Unterbeck, A., Salbum, J. M., Masters, C. L., Grzeschik K-H., Multhaup, G., Beyreuther, K. and Müller-Hill, B.